FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11900209
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT42850.018.0364931
CTTCCGA69300.017.823981
TATGCCG20000.017.61429848
CGTATGC20600.017.1083946
GTATGCC21400.016.95120247
CTAGCAG44400.016.618158
CCCTACA17550.016.30627310
ACTCTAG51000.015.6867715
CCGATCT77800.015.5132354
CGGGGGG9900.015.3345991
GACTCTA54800.014.53601554
TCCCTAC21650.014.333049
CTGACTC56450.013.8063382
CGCGGGG7750.013.8011371
CTATCGA54300.013.20822917
TCTATCG56400.012.89951816
TCGTATG29100.012.82410545
CGGGAAT94950.012.7909441
GCACACG34350.012.64729111
TTCCGAT97550.012.5851012
Produced by FastQC (version 0.11.2)