FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14854176
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT56350.020.3257681
CTAGCAG59450.018.2113538
ACTCTAG66600.017.552945
CCCTACA23500.017.31348410
GACTCTA68450.017.0281584
TATGCCG22500.015.50127948
CTGACTC70150.015.3871652
CGTATGC23550.015.25657646
TCCCTAC27450.015.073289
CTTCCGA82750.015.0085011
GTATGCC24300.014.783647
CTATCGA72850.014.29461817
TCTATCG75950.013.93784216
TAGCAGA82250.013.6662869
GCACACG40350.013.58700911
CTCTAGC89000.013.2127036
CCGATCT94700.012.85432154
CTAGCAT82750.012.58355626
TCTAGCA124100.012.502747
CGCGGGG9400.012.4782561
Produced by FastQC (version 0.11.2)