FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14617978
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26650.023.45483648
GTATGCC27000.023.15832947
CGTATGC28750.021.39092846
CGTACGT30600.020.87209134
GTACGTA33400.018.71099535
GCTGACT49450.018.000891
CTTCCGA84100.017.9687841
CCGTACG36250.017.8057433
TACGTAA36900.017.2197836
TCGTATG37900.017.1389445
CTCGTAT37750.016.0173644
CTAGCAG54250.016.0157998
CCCTACA20750.015.95153410
CCGATCT92800.015.9043684
GCACACG50050.015.15547111
ACCGTAC45000.014.57172632
ACTCTAG62950.014.5167465
ACGTCTG53750.014.30518715
GACTCTA66850.014.0825674
CGGGGGT13100.013.69533351
Produced by FastQC (version 0.11.2)