FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965570
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG81940.11763574265996897No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC80670.11581248914302779No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC74260.10661008359689157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13700.024.4463848
CGTATGC13350.024.3218946
GCTGACT28900.020.1772561
GTATGCC16600.019.97409247
CTAGCAG28750.019.9101838
CGCGGGG3700.019.583521
ACTCTAG31200.019.3439245
TCGTATG17550.018.50179945
GCACACG24650.018.04573811
GACTCTA35550.018.044074
CTTCCGA35700.017.4937761
ACACGTC25500.017.30923713
CTCGTAT18850.017.22581344
GTCACGT20450.017.20317329
CACACGT25800.017.10772112
CTGACTC34800.017.0461352
ACGTCTG27000.016.47544715
AGTCACG21800.016.29347828
CGGGGGT6900.016.0019931
GCGGGGG6900.016.0019931
Produced by FastQC (version 0.11.2)