FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11880503
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC123850.10424642795006239No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG122550.1031521981855482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28500.028.59644748
CGTATGC27950.028.30716546
GTATGCC32150.024.82044847
TCGTATG35850.022.64870645
GTCACGT37600.021.7402229
TCACGTG38150.021.70240430
GGCCTTA37800.021.2849436
CTCGTAT38650.020.5610344
GCCTTAT39600.020.408637
CGTGGCC39700.020.25678833
GCACACG46250.019.90516711
ACGTGGC41500.019.70869432
AGTCACG41050.019.5731328
GCCGTCT41400.019.41525751
TATCTCG40700.019.3553741
CCCTACA16300.019.24952910
ACGTCTG48000.019.10826915
CACACGT50050.018.80723212
CTTCCGA66850.018.7862681
CACGTGG45000.018.47944831
Produced by FastQC (version 0.11.2)