FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_tric-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_tric-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35448791
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG988200.27876832245139194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266400.032.1823041
GTATCAA507850.027.9547711
AACGCAG599800.022.303216
CAACGCA597600.022.1842525
TCAACGC601700.022.0799834
CGCAGAG617800.021.6044798
ACGCAGA623350.021.3837287
GCTTACG113200.021.1617262
AGAGTAC630150.021.15458511
ATCAACG633300.021.1330173
CTTACGC116850.020.964323
CAGAGTA669000.020.0598110
TTACGCC122850.019.7979034
GCAGAGT679500.019.7090539
TACGCCG124600.019.5743055
TATCAAC698750.019.5668582
GGCTTAC127150.019.5055261
GAGTACG517650.019.32080512
GTACGGG520600.019.15170714
AGTACGG520300.019.13625313
Produced by FastQC (version 0.11.2)