FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_trib.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_trib.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26969415
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239850.038.7507131
GTATCAA531950.031.4773041
TCAACGC624500.025.2064744
CAACGCA629600.024.9465755
ATCAACG637300.024.8267863
AACGCAG643350.024.782796
CGCAGAG669650.024.1201978
ACGCAGA663550.023.9928867
AGAGTAC697650.022.99952711
GCAGAGT715800.022.4835729
TATCAAC719350.022.4367282
CAGAGTA742700.021.72966210
AGTACGG433950.021.01216913
GAGTACG438900.020.9402612
GCTTACG75900.020.555212
GTACGGG445950.020.51634214
CTTACGC76550.020.2813873
TACGGGG304600.020.10389915
GAGTACT351550.019.64673612
TACGCCG81650.019.2235345
Produced by FastQC (version 0.11.2)