FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_tria.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_tria.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23148873
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186750.038.6845171
GTATCAA417450.031.0049631
TCAACGC483550.025.2410624
CAACGCA483600.025.180575
AACGCAG494400.024.9998846
ATCAACG496750.024.6014143
CGCAGAG514800.024.0177278
ACGCAGA513350.023.9099127
AGAGTAC535800.023.10293611
TATCAAC546500.022.8498122
GCAGAGT550300.022.531379
CAGAGTA559600.022.23104510
GAGTACG338450.021.0495612
AGTACGG335450.020.92936113
GTACGGG343750.020.63455214
TACGGGG238100.020.53166615
GCTTACG48800.020.4400632
CTTACGC50700.020.279523
GGCTTAC56600.019.585051
GAGTACT278900.018.93843812
Produced by FastQC (version 0.11.2)