FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_shf-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_shf-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32032469
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACGCCGGTCTGAACTCAGCTCATGTAAAGTTTTAAGGGTTGAACAAACCCACTTTTAAGGTTGCTGCACC500760.15632887992492867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328450.042.5691681
GTATCAA713300.031.9494931
GCTTACG165650.027.2968142
CTTACGC170800.026.7243673
TACGCCG171600.026.5158375
TTACGCC174700.025.9868534
GGCTTAC181850.025.5845741
CCGGTCT178150.025.42619
CGCCGGT177650.025.3215077
GCCGGTC181450.024.9632138
TCAACGC889100.024.6307134
CAACGCA894000.024.5335755
AACGCAG906800.024.4147596
ATCAACG902900.024.329363
ACGCCGG191500.023.6695966
CGCAGAG948000.023.5044278
ACGCAGA942650.023.4174067
TATCAAC983000.022.7256892
CGGTCTG199450.022.69349110
TTAAGGG199250.022.57842637
Produced by FastQC (version 0.11.2)