FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_sha2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_sha2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24220702
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214500.038.930391
GTATCAA459750.031.4439581
TCAACGC557600.024.7358464
CAACGCA559600.024.5605225
AACGCAG572750.024.387286
ATCAACG573650.024.1178763
GCTTACG65150.023.9984552
CTTACGC67450.023.9389763
CGCAGAG599750.023.3994568
ACGCAGA604900.022.9376097
TATCAAC624050.022.5652582
TACGCCG71000.022.4966895
TTACGCC72000.022.1847864
AGAGTAC631750.021.97479411
GCAGAGT642400.021.7871699
GGCTTAC76000.021.175421
CAGAGTA671750.020.77379210
CCGGTCT78950.020.1879029
CGCCGGT77900.020.0166157
GAGTACT294250.019.72074912
Produced by FastQC (version 0.11.2)