FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_ld-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_ld-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30524367
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274700.038.0585671
GTATCAA576100.031.3546891
TCAACGC702450.024.526144
CAACGCA707550.024.4021515
AACGCAG713800.024.3717786
GCTTACG90150.024.2744142
ATCAACG723150.023.9460183
CTTACGC95650.023.3380093
CGCAGAG751800.023.2284878
ACGCAGA749500.023.1333457
AGAGTAC770850.022.5566111
TATCAAC784450.022.3875772
TTACGCC99800.022.330484
GGCTTAC103150.022.2999381
GCAGAGT801850.021.8993249
TACGCCG104600.021.8656835
CCGGTCT103250.021.5179989
CAGAGTA824100.021.2747110
CGCCGGT107100.020.3892777
GAGTACG568000.019.89205712
Produced by FastQC (version 0.11.2)