FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_lc-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_lc-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33620317
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG825790.24562231224649073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271350.036.9272731
GTATCAA565200.028.5332371
TCAACGC667250.022.903864
AACGCAG679350.022.6267346
CAACGCA677700.022.5449585
ATCAACG696150.022.0692043
CGCAGAG699600.021.9830028
ACGCAGA702050.021.8320267
AGAGTAC743850.020.6249111
GCAGAGT759700.020.3031679
TATCAAC793600.019.760192
CAGAGTA789200.019.5793310
GCTTACG84750.019.102192
CTTACGC89800.018.9796143
GAGTACG487750.018.22762312
AGTACGG488000.018.16887913
TTACGCC93600.018.0229784
TACGCCG93600.018.0224695
GTACGGG498550.017.80505614
GGCTTAC101700.017.5250021
Produced by FastQC (version 0.11.2)