FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_la.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_la.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37764416
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA522250.045.7152671
GTATCAA1107050.034.600041
GCTTACG130250.031.8524322
CTTACGC131750.031.6605033
TTACGCC136550.030.4942554
GGCTTAC145800.029.6801911
TACGCCG142400.029.3857775
CCGGTCT149450.028.3931779
CGCCGGT148900.027.5700827
CAACGCA1409150.026.4615125
TCAACGC1417100.026.3116044
AACGCAG1446750.026.0090686
ATCAACG1444350.025.8653283
TATCAAC1477650.025.6195622
GCCGGTC166800.025.5635328
ACGCAGA1521650.024.6320617
CGCAGAG1557850.024.2574718
GCAGAGT1558950.024.2230059
AGAGTAC1563250.024.05754511
ACGCCGG172600.024.0435186
Produced by FastQC (version 0.11.2)