FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_atc-1-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_atc-1-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31774798
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACGCCGGTCTGAACTCAGCTCATGTAAAGTTTTAAGGGTTGAACAAACCCACTTTTAAGGTTGCTGCACC372740.11730680396457595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA261250.037.2813571
GTATCAA539300.029.907861
GCTTACG130400.025.6748832
CTTACGC134450.025.2763733
TTACGCC141200.024.2611964
TCAACGC630650.024.1840924
TACGCCG143050.024.0912635
CAACGCA632950.024.0898885
AACGCAG641050.023.9999226
GGCTTAC147100.023.6202351
ATCAACG652800.023.4873093
CCGGTCT148450.023.1228439
ACGCAGA666950.023.0218077
CGCAGAG673200.022.9523478
CGCCGGT148850.022.6191397
AGAGTAC700000.021.89228611
GCCGGTC157200.021.813438
GCAGAGT716900.021.5825219
TATCAAC731100.021.3910272
ACGCCGG161250.021.2216346
Produced by FastQC (version 0.11.2)