FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_atb-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_atb-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30889291
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597850.1935460415714948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249700.036.0466651
GTATCAA501650.029.9431721
CAACGCA598800.023.8220375
TCAACGC602800.023.6593864
AACGCAG609200.023.6471826
ATCAACG623250.023.0798423
CGCAGAG627300.022.8611648
ACGCAGA631650.022.6651297
TATCAAC683600.021.3798872
AGAGTAC671900.021.3701311
GCAGAGT674000.021.3183529
CTTACGC76950.020.2671643
CAGAGTA715900.020.18651810
GCTTACG73950.019.931792
GAGTACG488250.019.57973712
AGTACGG487450.019.51281713
TTACGCC82850.018.863054
TACGCCG81850.018.83975
GTACGGG509550.018.68008614
TACGGGG365350.018.46084815
Produced by FastQC (version 0.11.2)