FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n02_ata.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n02_ata.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23455050
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190150.035.9108121
GTATCAA387900.030.8530711
TCAACGC466300.024.3404924
CAACGCA472250.024.0400495
AACGCAG479650.023.9922436
GCTTACG81950.023.6286932
ATCAACG486550.023.4154323
CTTACGC85150.023.2989223
CGCAGAG494950.023.2661658
ACGCAGA502200.022.922637
TTACGCC88550.022.3236354
TACGCCG90400.022.0184545
AGAGTAC528100.021.85833211
TATCAAC537700.021.6457842
GCAGAGT537900.021.4599579
GGCTTAC94500.021.454641
CGCCGGT93750.020.8639627
CCGGTCT96250.020.7889089
CAGAGTA559900.020.78314410
AGTACGG394600.019.88993613
Produced by FastQC (version 0.11.2)