FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_tric-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_tric-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35448791
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263350.031.966891
GTATCAA501800.027.9953351
CAACGCA620550.021.9708775
AACGCAG630600.021.779456
TCAACGC626550.021.7162674
CGCAGAG646500.021.1429568
ACGCAGA655450.020.8647237
ATCAACG656700.020.8556863
GCTTACG106500.020.8012052
CTTACGC110500.020.7043533
AGAGTAC663750.020.65541311
CAGAGTA704550.019.5279910
TTACGCC117550.019.4627864
TATCAAC708950.019.4448362
GCAGAGT713950.019.2952339
TACGCCG121300.019.2879495
GAGTACG508000.018.86583512
GGCTTAC121900.018.7729171
TAAGGCG139400.018.7343329
AGTACGG514750.018.69216713
Produced by FastQC (version 0.11.2)