FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_trib.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_trib.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26969415
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234250.037.7619131
GTATCAA503400.030.7835081
TCAACGC638700.024.3277654
CAACGCA643150.024.3258515
AACGCAG654000.024.0912536
ATCAACG650350.023.9236723
CGCAGAG684850.023.122718
ACGCAGA689250.022.8648957
AGAGTAC715900.022.0760111
GCAGAGT731800.021.6533329
CTTACGC74000.021.2165363
TATCAAC714400.021.203652
CAGAGTA760200.020.86681410
GCTTACG71050.020.8343892
TACGCCG79050.019.9486335
CCGGTCT81900.019.840739
GAGTACG441250.019.7966112
TTACGCC79250.019.7675914
AGTACGG440200.019.66346513
GGCTTAC83000.019.4188631
Produced by FastQC (version 0.11.2)