FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_tria.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_tria.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23148873
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182300.036.972461
GTATCAA395900.030.0917911
CAACGCA490900.024.4820185
TCAACGC489350.024.4537944
AACGCAG498900.024.2417586
ATCAACG499550.023.8714493
ACGCAGA523750.023.1276057
CGCAGAG532000.022.8921728
AGAGTAC555250.021.89585111
TATCAAC536650.021.6355782
GCAGAGT571000.021.3407389
CAGAGTA578850.021.0628410
GAGTACG339700.019.7532512
AGTACGG343600.019.42855313
GTACGGG349350.019.11844414
TACGGGG238750.019.03042415
GAGTACT298300.018.28492412
CTTACGC50600.017.5935363
TACGCCG55550.017.4545635
GCTTACG48250.016.8767662
Produced by FastQC (version 0.11.2)