FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_shf-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_shf-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32032469
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACGCCGGTCTGAACTCAGCTCATGTAAAGTTTTAAGGGTTGAACAAACCCACTTTTAAGGTTGCTGCACC501480.15655365185868125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA317300.042.749561
GTATCAA690100.032.0043641
GCTTACG156450.025.6954232
CTTACGC160550.025.5123543
TACGCCG165550.024.99235
TTACGCC166100.024.5769084
TCAACGC907400.024.2966444
CAACGCA912950.024.2436625
AACGCAG926050.024.1504846
ATCAACG915900.024.1237933
CCGGTCT172450.024.0669869
CGCCGGT170300.024.0669147
GCCGGTC173300.023.8892948
GGCTTAC174750.023.4643421
CGCAGAG968650.023.0725028
ACGCAGA973450.022.9090087
ACGCCGG185000.022.3461846
TATCAAC985450.022.196862
AGAGTAC1016250.021.92024811
TTAAGGG186150.021.83199337
Produced by FastQC (version 0.11.2)