FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_sha2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_sha2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24220702
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216550.038.695031
GTATCAA454000.030.8095551
TCAACGC579200.024.1868214
CAACGCA583750.024.1638035
AACGCAG595050.023.9136836
ATCAACG600050.023.4152573
GCTTACG61150.023.1909522
CGCAGAG631500.022.5955548
ACGCAGA636450.022.3114977
CTTACGC64600.022.2731743
TATCAAC638800.021.4544582
TTACGCC68000.021.3626174
TACGCCG69300.021.36025
AGAGTAC669550.021.25958811
GCAGAGT674500.021.2061279
GGCTTAC74000.020.706941
CAGAGTA711550.020.13082110
CGCCGGT72300.019.9937067
GAGTACT326000.019.72594812
CCGGTCT77300.019.4590239
Produced by FastQC (version 0.11.2)