FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_ld-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_ld-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30524367
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273300.038.1633341
GTATCAA576350.031.780031
CAACGCA743700.024.5079755
TCAACGC742600.024.3955024
AACGCAG751300.024.3612216
ATCAACG760300.023.8773353
GCTTACG85550.023.3131582
CTTACGC88850.023.185663
CGCAGAG796050.023.060768
ACGCAGA795200.023.0074317
AGAGTAC822150.022.3742111
TATCAAC809700.022.2155422
GCAGAGT846500.021.7800839
TTACGCC94950.021.4418584
CAGAGTA877450.021.05467610
TACGCCG99950.021.0252575
GGCTTAC100950.020.7526651
CCGGTCT101350.020.5945199
CGCCGGT102350.019.955237
GAGTACG579550.019.66187112
Produced by FastQC (version 0.11.2)