FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_lc-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_lc-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33620317
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274450.037.527531
GTATCAA572650.028.9191441
TCAACGC708450.022.7951894
CAACGCA715750.022.6206195
AACGCAG719800.022.5461436
ATCAACG733200.022.1385973
CGCAGAG748100.021.6933358
ACGCAGA748850.021.6622987
AGAGTAC800850.020.25553311
GCAGAGT807700.020.1395749
TATCAAC825300.019.5213642
CAGAGTA842600.019.35840410
GCTTACG82500.019.3235172
CTTACGC84100.019.2844433
TTACGCC87450.018.6641354
TACGCCG87900.018.6471565
CCGGTCT92600.018.1814089
AGTACGG501350.017.93261713
GAGTACG502000.017.8956812
GTACGGG511450.017.48392514
Produced by FastQC (version 0.11.2)