FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_la.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_la.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37764416
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478540.12671717205953878No Hit
GGCTTACGCCGGTCTGAACTCAGCTCATGTAAAGTTTTAAGGGTTGAACAAACCCACTCTTAAGGTTGCTGCACC383320.1015029598233427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA541200.046.3111951
GTATCAA1129500.034.051981
CTTACGC125900.031.07983
GCTTACG125550.030.8911552
TACGCCG135450.030.2135095
TTACGCC131450.029.9778064
GGCTTAC135950.029.8802871
CGCCGGT143500.027.7926967
CCGGTCT148700.027.7488129
CAACGCA1537350.025.7917375
TCAACGC1537100.025.5983124
GCCGGTC160000.025.5734928
AACGCAG1572700.025.3527326
ATCAACG1561100.025.2487753
TATCAAC1559500.024.5839862
ACGCCGG168050.024.024156
ACGCAGA1681400.023.7157237
CGCAGAG1711200.023.3995298
GCAGAGT1719550.023.313939
AGAGTAC1730200.023.11809211
Produced by FastQC (version 0.11.2)