FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_atc-1-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_atc-1-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31774798
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTACGCCGGTCTGAACTCAGCTCATGTAAAGTTTTAAGGGTTGAACAAACCCACTTTTAAGGTTGCTGCACC361430.11374737929097142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA260400.037.019751
GTATCAA534650.029.8763311
GCTTACG123550.024.883962
CTTACGC128950.024.6718083
TCAACGC655500.024.1515394
CAACGCA659400.024.1396355
AACGCAG667100.024.016336
TTACGCC134800.023.4220734
ATCAACG679400.023.3982873
TACGCCG138650.022.9460125
ACGCAGA697700.022.9390417
GGCTTAC139250.022.9271471
CGCAGAG701600.022.9050678
CCGGTCT142950.022.4450789
AGAGTAC738850.021.79666511
CGCCGGT145200.021.5981667
GCAGAGT751450.021.440719
TATCAAC746350.021.1048762
GCCGGTC152700.020.7859768
CAGAGTA788550.020.52801710
Produced by FastQC (version 0.11.2)