FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_atb-corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_atb-corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30889291
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA252250.036.024971
GTATCAA501350.029.3672811
CAACGCA619650.023.5776465
TCAACGC619600.023.4513844
AACGCAG629050.023.346156
ATCAACG643600.022.6947173
CGCAGAG654300.022.3726968
ACGCAGA661250.022.1218997
AGAGTAC702150.020.9999911
GCAGAGT705450.020.823819
TATCAAC705000.020.492742
CAGAGTA746400.019.82906510
CTTACGC74000.019.3046153
AGTACGG488050.019.11410913
GAGTACG489500.019.08577212
GCTTACG74450.018.4460982
TACGGGG354650.018.4241515
GTACGGG508700.018.29063414
GAGTACT294000.018.25895912
TACGCCG81500.017.9093075
Produced by FastQC (version 0.11.2)