FastQCFastQC Report
Fri 4 May 2018
HLNVLBGX5_n01_ata.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVLBGX5_n01_ata.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23455050
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199450.037.327151
GTATCAA387050.029.6862581
TCAACGC492050.023.0238424
AACGCAG501100.022.9180586
CAACGCA497900.022.8781285
GCTTACG80150.022.3014492
ATCAACG511750.022.2855433
CTTACGC84300.022.1861213
CGCAGAG527950.021.7737838
ACGCAGA528750.021.6884567
TTACGCC87450.021.3083634
TACGCCG88300.021.2205285
AGAGTAC558600.020.67749411
TATCAAC547750.020.6191372
GGCTTAC91000.020.5193981
GCAGAGT571100.020.2554639
CGCCGGT91650.019.7626327
CAGAGTA591500.019.53350610
CCGGTCT96450.019.2800129
GAGTACG407550.018.90266412
Produced by FastQC (version 0.11.2)