FastQCFastQC Report
Wed 18 May 2022
HLNVGAFX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVGAFX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190350423
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGACAGTC18199034295.60805756654399No Hit
AGATCTCGGT30135351.5831511968849157Illumina Single End PCR Primer 1 (100% over 10bp)
AGCTCTCGGT19720011.0359845641110028No Hit
GGGGGGGGGG4848320.2547049764107958No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)