FastQCFastQC Report
Wed 18 May 2022
HLNVGAFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNVGAFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190350423
Sequences flagged as poor quality0
Sequence length10
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCACCA17829029393.66424838467525No Hit
GGGGGGGGGG60481623.177383010070852No Hit
ACCGCACCAA5575550.29290977724803896No Hit
CACGCACCAA5334310.28023630922007464No Hit
CACCGCACAA4738810.2489519027756508No Hit
GCCCCCCCCC3887410.2042238697835728No Hit
CACCCACCAA2608490.13703620716408915No Hit
CCCGCACCAA2288870.1202450703248503No Hit
CACCGACCAA1955630.10273841104098834No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)