FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17573636
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6453853.6724614075311446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA21150.022.53656219
CTAACGT21700.019.9097711
TAAGAGT53900.019.50733220
ACACGGG15550.018.93932225
AATAGTG51850.018.93610456
AGATCGG34650.018.6524331
TAGGGAA26400.018.5861520
CACGGGA15850.018.58239226
TTGTAAG59150.018.13168717
TCGTGTA31200.018.08461815
TGTAAGA60450.017.45230918
AAGAGTG28100.017.2196526
GCCAGTG23350.017.06415253
AGTGAAT61600.016.80640859
AGAAAGT64500.016.7253769
TGTTAAG63300.016.49371543
CTCAACT29450.016.3401551
TAGTGAA62050.016.33919358
TACCGGA28900.016.1190229
CTCCGAA20450.015.9738851
Produced by FastQC (version 0.11.5)