FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032700
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC25500.24692553500532585No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC25360.24556986540137504No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG19740.19114941415706402No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA19460.1884380749491624No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA18320.17739905103127723No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT18280.17701171685871986No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA16620.16093734869758886No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT16350.15832284303282657No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG16010.15503050256608888No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA15980.15474000193667084No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC15280.14796165391691682Illumina Single End PCR Primer 1 (97% over 35bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA15030.14554081533843324No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC14720.14253897550111358No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG14500.14040863755204802No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT14220.1376972983441464No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG14070.13624479519705626No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG13400.12975694780672026No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT13240.12820761111649076No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT12940.12530260482231045No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA12780.12375326813208096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12540.12142926309673671No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG12340.11949259223394985No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA10980.10632323036699914No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG10800.10458022659049093No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA10700.10361189115909751No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG10470.10138471966689262No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT10340.10012588360608114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC4000.056.87318853
GTGGTCG4400.056.47547554
TCGGTGG3950.055.82100751
CTATAGT3700.054.8631237
CGGTGGT4250.052.70420552
GTAGATC3250.049.53208544
GCTATAG4050.049.25530636
TAGTGTA4050.048.3958740
TGTAGGC5100.048.04001631
ATAGTGT4150.047.22970239
GTCGCCG4850.046.18409757
GGTCGCC5250.045.99630756
GTCGTGT6850.045.98616414
TATAGTG4200.044.9985738
CGTGTAG6050.044.54619616
GTAGGGA5900.044.49442319
TCTCGGT4100.044.38883249
TCGTGTA7150.043.56715415
TAGATCT3950.043.41213245
CTCTAGT4400.042.953186
Produced by FastQC (version 0.11.5)