FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T3_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T3_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130947
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC20360.1800261197032222No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG16820.14872491814382105No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC16660.1473101745705148No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA15290.1351964327240799No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG14650.12953745843085485No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC13030.11521317975112892No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA12480.11034999871788864No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA12380.10946578398457223No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT12010.10619418947130149No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA11610.10265733053803582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT2100.056.6575437
CGGTGGT2550.053.53735752
GTAGGAT2600.052.48922732
GGTGGTC2650.051.5216453
GATAGGG2400.049.57315436
TAGGATA2800.048.7399933
TCGGTGG2800.045.00668351
TAGGGTG2750.043.2657638
GTAGGGA3550.042.3912919
GGGTGTA2850.041.74950440
AGAGTGT3250.039.83797527
GTGTAGA3100.039.51325642
GGTCGCC3600.038.898156
GTGGTCG3700.037.84680654
GGTGTAG3150.037.77503641
GAGTGTA3550.035.48567228
TAGGGAA4200.034.99745620
CGTGTAG4350.033.7906516
AGGATAG3450.033.4713934
CTCTAGT2850.031.9246796