FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901905
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC40670.4509344110521618Illumina Single End PCR Primer 1 (96% over 33bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC17620.19536425676761968No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC14530.16110344215854222No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT14410.15977292508634502No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG13700.15190069907584502No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA12550.1391499104672887No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA12400.1374867641270422No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT11590.1285057738897112No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA10840.12019004218847883No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT10830.12007916576579572No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG10770.11941390722969715No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA10570.11719637877603518No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA10310.11431359178627461No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG10240.11353745682749293No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT10030.11120905195114786No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG9950.11032204056968306No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA9160.10156280317771826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT5500.061.08519752
TCGGTGG5450.060.36133251
GGTGGTC5350.059.52714553
GGTCGCC5400.057.03170456
GTGGTCG5750.055.38612754
AGATCTC5550.052.9680246
GTGTAGA5700.052.1852142
CCTCTAT6300.051.6618435
GTGTGCC6600.051.44885330
GTGCCTC6750.051.3427732
GTAGATC5700.050.9601544
GCCTCTA6350.050.70392234
GTCGCCG6150.050.07662257
CTCGGTG5850.049.65347750
AGTGTGC6950.048.857929
CTCTATG6000.047.82621436
TAGATCT6300.047.21780845
ATCTCGG6450.047.2048948
GTAGGGA7900.046.96284519
TGGTCGC6950.046.32660355
Produced by FastQC (version 0.11.5)