FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682628
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAG150.002227389569.9772237
ATAGGGT850.053.5119937
GATAGGG900.050.53910436
TCGGTGG1200.049.59262551
GGTGGTC1550.040.6527853
CGGTGGT1650.040.31058552
TAGGGTG1150.039.55523738
GTAGGAT1700.039.1163832
GGTCGCC1800.038.89617556
TAGGATA1450.038.61660833
AGAGTGT1750.035.99883327
GTAGGGA1700.034.99373619
GGGTGTA1400.032.49180240
AGTGTAG1750.031.99896229
GTGTAGA1700.030.87682742
ATCTCGG1750.030.00562348
CGTGTAG2050.029.01919616
TAGATCT1601.8189894E-1228.44074445
ACTCTAG1601.8189894E-1228.432415
CTCTAGT1601.8189894E-1228.432416
Produced by FastQC (version 0.11.5)