Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_surE_T2_R2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 788581 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC | 7568 | 0.9596984964131775 | Illumina Single End PCR Primer 1 (96% over 33bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 1285 | 0.1629509207044045 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 815 | 0.10335019484364955 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGGTGG | 1050 | 0.0 | 65.346596 | 51 |
GGTGGTC | 1145 | 0.0 | 63.90754 | 53 |
GTGTAGA | 1145 | 0.0 | 62.648655 | 42 |
GTGGTCG | 1180 | 0.0 | 62.605392 | 54 |
CTCTATG | 1105 | 0.0 | 62.070423 | 36 |
GGTCGCC | 1185 | 0.0 | 62.041843 | 56 |
CGGTGGT | 1135 | 0.0 | 61.998898 | 52 |
TAGATCT | 1070 | 0.0 | 61.488304 | 45 |
GTAGATC | 1100 | 0.0 | 61.394295 | 44 |
AGATCTC | 1025 | 0.0 | 60.773552 | 46 |
TGTGTAG | 1165 | 0.0 | 60.672073 | 41 |
ATCTCGG | 1050 | 0.0 | 59.65986 | 48 |
GCCTCTA | 1160 | 0.0 | 59.432873 | 34 |
CCTCTAT | 1165 | 0.0 | 59.174038 | 35 |
GTGTGCC | 1250 | 0.0 | 59.073257 | 30 |
GTGCCTC | 1190 | 0.0 | 58.816814 | 32 |
CTCGGTG | 1070 | 0.0 | 58.56329 | 50 |
TGGTCGC | 1275 | 0.0 | 57.11688 | 55 |
TGTAGAT | 1290 | 0.0 | 56.69176 | 43 |
AGAGTGT | 1315 | 0.0 | 56.68195 | 27 |