FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788581
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC75680.9596984964131775Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12850.1629509207044045No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT8150.10335019484364955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG10500.065.34659651
GGTGGTC11450.063.9075453
GTGTAGA11450.062.64865542
GTGGTCG11800.062.60539254
CTCTATG11050.062.07042336
GGTCGCC11850.062.04184356
CGGTGGT11350.061.99889852
TAGATCT10700.061.48830445
GTAGATC11000.061.39429544
AGATCTC10250.060.77355246
TGTGTAG11650.060.67207341
ATCTCGG10500.059.6598648
GCCTCTA11600.059.43287334
CCTCTAT11650.059.17403835
GTGTGCC12500.059.07325730
GTGCCTC11900.058.81681432
CTCGGTG10700.058.5632950
TGGTCGC12750.057.1168855
TGTAGAT12900.056.6917643
AGAGTGT13150.056.6819527