FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surE_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surE_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692094
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC8900.128595248622297No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC8400.12137079645250502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG1500.051.3383751
GCTATAG1250.047.5943536
ATAGTGT1350.044.06884439
GGTGGTC1750.044.0043153
GTAGATC1600.043.7479744
CGGTGGT1850.043.51778452
CTATAGT1450.043.4431237
GGTCGCC1900.040.5273656
GTGGTCG1950.039.49105554
TAGTGTA1550.038.38253840
GTAGGGA3000.037.331619
TAGATCT1950.035.8983645
CTAGGTG400.002417606535.00343350
CGCCCTA501.7087962E-435.0034359
CGTGTAG3250.034.45744716
AGAGTGT2350.034.2537327
TGTAGAT2600.033.65228343
GAGTGTA2550.032.93964828
TGTAGGC2700.032.40590331
GTGTAGA1650.031.81440542
Produced by FastQC (version 0.11.5)