FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T4_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T4_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933151
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT31930.34217398898999196No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG26630.2853771790417628No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC21120.22632992945407548No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG20790.22279352430635557No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA16960.18174979183433335No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA15980.1712477401835287No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA14430.15463735236848056No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC14290.15313705927550847No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC14170.15185109376724668No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG13750.14735021448833038No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT11910.12763207669498292No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT11400.12216672328487028No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG11370.12184523190780486No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA11290.12098792156896365No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT11220.12023777502247761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10710.11477242161236499No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA10290.1102715423334487No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT10180.10909274061754208No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA9820.10523484409275669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT1550.056.45874452
GTGTAGA2000.054.2394142
GGTGGTC1800.052.50663853
GGGTGTA2050.049.5025340
GTAGGAT1950.048.46246732
ATAGGGT2050.047.7929837
CTAGGTG305.8751716E-446.6725650
TGTACTC8450.045.9772152
CTCTAGT8600.044.76356
GATAGGG2250.043.54472436
GTTACTA2050.042.6791653
GGTGTAG2300.042.6003841
TAGGATA2350.041.7006933
TCTAGTT9250.041.6179547
GTAGATC2200.041.3555644
ACTCTAG9650.039.890725
GGTCGCC2400.039.3757556
TCGGTGG2250.038.89380651
ACTACCC2250.038.8854686
CTAGTTG9550.038.8447468
Produced by FastQC (version 0.11.5)