FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784979
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC44730.5698241609011196Illumina Single End PCR Primer 1 (96% over 33bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC31860.4058707302997915No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA18530.23605727032188123No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT17450.22229894048121035No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA17410.22178937270933363No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT16860.214782815846029No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA15470.1970753357733137No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT15280.19465488885689936No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG15190.19350836137017677No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG14570.18561006090608792No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA14440.18395396564748867No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC14310.1822978703888894No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG13470.1715969471794787No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA13020.1658643097458658No Hit
GTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTT12900.1643356064302357No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA12770.16267951117163643No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT12190.1552907784794243No Hit
GATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCA11900.1515964121333182No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG11250.14331593584032185No Hit
CCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACA11180.14242419223953762No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC11010.14025852920906165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10970.13974896143718493No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA10800.13758329840670896No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG10630.135417635376233No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA10610.13516285149029464No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA10290.13108630931528106No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC10010.12751933491214415No Hit
CGGGGAGGTAGCGGTGCCCTGTACCTGCAATCCGCTCTAGCAGGGCCGAA9870.1257358477105757No Hit
GTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTG9420.12000321027696283No Hit
CGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGC9420.12000321027696283No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA9060.11541710033007252No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATC9030.11503492450116501No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA8830.11248708564178149No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG8680.11057620649724388No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC8540.10879271929567544No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG8150.1038244335198776No Hit
CGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTAC7990.1017861624323708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC7800.066.8624453
GGTCGCC8350.065.3884556
TCGGTGG7450.064.3657651
CGGTGGT8050.063.9163652
GTGTAGA7450.063.4180342
GTAGATC7100.061.61506344
GTGGTCG8850.060.90711654
TGTAGAT8850.059.31755443
GCCTCTA8750.059.1955334
GTGCCTC8800.058.46149432
GTCGCCG9400.058.0881257
TGGTCGC9400.058.0881255
CCTCTAT9050.058.00666835
TAGATCT7950.057.2283845
GTGTGCC9900.056.9148930
AGAGTGT9650.056.2133727
AGTGTGC9950.055.92542329
CCGTATC9200.055.5463661
CTCGGTG7500.055.06952350
CTCTATG8400.054.57921236
Produced by FastQC (version 0.11.5)