FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461025
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC37800.25872247223695694No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC36830.25208329768484455No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA28220.1931520678975377No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC26280.1798737187933129No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG26110.17871015211923136No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA25540.17480878150613438No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT25170.17227631286254513No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG23270.1592717441522219No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA23080.15797128728118956No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG22700.15537037353912492No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG22530.15420680686504337No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT21720.14866275388853717No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA21230.14530894406324327No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA21180.14496671857086635No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA21120.14455604798001404No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA20320.1390804401019832No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT18580.12717099296726614No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT18240.12484385961910303No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA17620.12060026351362912No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT17310.11847846546089219No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG16360.11197618110573057No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA15940.10910148696976438No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG15380.1052685614551428No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA15180.10389965948563509No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC14740.10088807515271812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG2850.052.81424751
CTATAGT2800.051.24473637
CGGTGGT3250.050.6223252
GCTATAG2900.048.2659536
GGTGGTC3700.048.25150353
GGTCGCC4100.046.95765356
AGAGTGT4600.045.64748427
TAGTGTA3300.045.60293640
ATAGTGT3250.045.22767339
GTAGGGA5450.044.9495119
TGTAGGC5050.044.3518831
GTCGTGT6900.041.0827414
CGTGTAG6050.041.07016416
TAGGTGG953.6379788E-1240.53186851
TCGTGTA7050.039.71223415
GTGTAGA3400.039.11630642
TATAGTG3550.036.4763838
CTCTAGT4750.036.102796
GAGTGTA5900.035.58956528
GTGGTCG5350.035.3331554
Produced by FastQC (version 0.11.5)