FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T3_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T3_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128160
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC12610.11177492554247624No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC12340.10938164799319246No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT12310.10911572826549426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT2050.054.6340837
TCGGTGG1900.051.58345451
GATAGGG2150.050.45610436
TAGGGTG2250.049.77109538
CGGTGGT2150.048.8414852
GGTGGTC2450.048.57998353
GTAGGAT2300.047.1696732
TAGGATA2900.042.23570633
GGGTGTA2900.041.02897340
GTGTAGA3000.040.8296642
GGTCGCC2950.040.344356
GGTGTAG3100.039.51257741
AGGATAG2550.038.42621234
GTCGTGT4100.038.40947714
GAGTGTA3050.037.86544828
CGTGTAG3400.037.0538516
GTAGGGA3400.036.02457419
GTAGATC2750.035.6331644
AGAGTGT3300.034.99685327
AGTGTAG3100.034.99685329
Produced by FastQC (version 0.11.5)