FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807207
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC137791.706997089965771Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16540.20490407045528594No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT11170.1383783837355226No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA10570.1309453461132027No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC9900.12264512076827878No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA9860.12214958492679077No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT9830.12177793304567477No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT9360.11595538690819085No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA9330.11558373502707485No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA9130.11310605581963487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA18700.065.69256642
GGTGGTC18750.065.3307353
CTCTATG18250.065.19884536
GGTCGCC19150.065.0626856
CGGTGGT19050.065.0367752
GTCGCCG18650.064.9384457
GTAGATC17450.064.78252444
AGATCTC16750.064.35564446
GTGCCTC18800.064.0360432
AGTGTGC19900.063.66580629
TCGGTGG18950.063.53309251
GTGGTCG19800.063.28030854
TAGATCT17850.062.74259645
GTGTGCC20250.062.7382430
CCTCTAT18750.062.7136135
TGGTCGC19900.062.434755
TCTATGT19100.062.121837
TGTAGAT20200.062.02723343
CCGTATC18450.061.85568261
GTAGGGA21050.061.35147519