Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_surD_T3_R2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 807207 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC | 13779 | 1.706997089965771 | Illumina Single End PCR Primer 1 (96% over 33bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 1654 | 0.20490407045528594 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 1117 | 0.1383783837355226 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 1057 | 0.1309453461132027 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 990 | 0.12264512076827878 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 986 | 0.12214958492679077 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 983 | 0.12177793304567477 | No Hit |
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT | 936 | 0.11595538690819085 | No Hit |
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA | 933 | 0.11558373502707485 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 913 | 0.11310605581963487 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGTAGA | 1870 | 0.0 | 65.692566 | 42 |
GGTGGTC | 1875 | 0.0 | 65.33073 | 53 |
CTCTATG | 1825 | 0.0 | 65.198845 | 36 |
GGTCGCC | 1915 | 0.0 | 65.06268 | 56 |
CGGTGGT | 1905 | 0.0 | 65.03677 | 52 |
GTCGCCG | 1865 | 0.0 | 64.93844 | 57 |
GTAGATC | 1745 | 0.0 | 64.782524 | 44 |
AGATCTC | 1675 | 0.0 | 64.355644 | 46 |
GTGCCTC | 1880 | 0.0 | 64.03604 | 32 |
AGTGTGC | 1990 | 0.0 | 63.665806 | 29 |
TCGGTGG | 1895 | 0.0 | 63.533092 | 51 |
GTGGTCG | 1980 | 0.0 | 63.280308 | 54 |
TAGATCT | 1785 | 0.0 | 62.742596 | 45 |
GTGTGCC | 2025 | 0.0 | 62.73824 | 30 |
CCTCTAT | 1875 | 0.0 | 62.71361 | 35 |
TGGTCGC | 1990 | 0.0 | 62.4347 | 55 |
TCTATGT | 1910 | 0.0 | 62.1218 | 37 |
TGTAGAT | 2020 | 0.0 | 62.027233 | 43 |
CCGTATC | 1845 | 0.0 | 61.855682 | 61 |
GTAGGGA | 2105 | 0.0 | 61.351475 | 19 |