Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_surD_T3_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 753955 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 1445 | 0.19165600068969635 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 918 | 0.12175792984992473 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 908 | 0.12043159074480574 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC | 815 | 0.10809663706719896 | Illumina Single End PCR Primer 1 (97% over 35bp) |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 796 | 0.10557659276747286 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 778 | 0.10318918237825865 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 777 | 0.10305654846774674 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTCGCC | 230 | 0.0 | 54.77944 | 56 |
TCGGTGG | 190 | 0.0 | 53.417965 | 51 |
CGGTGGT | 205 | 0.0 | 52.92377 | 52 |
CTATAGT | 200 | 0.0 | 50.747066 | 37 |
TGTAGGC | 275 | 0.0 | 49.64008 | 31 |
GCTATAG | 205 | 0.0 | 49.509335 | 36 |
ATAGTGT | 225 | 0.0 | 48.216236 | 39 |
TAGTGTA | 225 | 0.0 | 48.216236 | 40 |
GGTGGTC | 255 | 0.0 | 46.66397 | 53 |
AGAGTGT | 290 | 0.0 | 45.8655 | 27 |
GTAGGGA | 310 | 0.0 | 45.15868 | 19 |
GTGTAGA | 195 | 0.0 | 44.86325 | 42 |
GTGGTCG | 300 | 0.0 | 41.997574 | 54 |
GTAGATC | 205 | 0.0 | 40.970524 | 44 |
TGTACTC | 315 | 0.0 | 40.013615 | 2 |
CTAGGTG | 35 | 0.0012558509 | 39.99769 | 50 |
CGTGTAG | 360 | 0.0 | 38.886642 | 16 |
CTCTAGT | 325 | 0.0 | 38.772133 | 6 |
AAGAGTG | 355 | 0.0 | 38.453583 | 26 |
TATAGTG | 275 | 0.0 | 38.17708 | 38 |