FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753955
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC14450.19165600068969635No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA9180.12175792984992473No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA9080.12043159074480574No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC8150.10809663706719896Illumina Single End PCR Primer 1 (97% over 35bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT7960.10557659276747286No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT7780.10318918237825865No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA7770.10305654846774674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2300.054.7794456
TCGGTGG1900.053.41796551
CGGTGGT2050.052.9237752
CTATAGT2000.050.74706637
TGTAGGC2750.049.6400831
GCTATAG2050.049.50933536
ATAGTGT2250.048.21623639
TAGTGTA2250.048.21623640
GGTGGTC2550.046.6639753
AGAGTGT2900.045.865527
GTAGGGA3100.045.1586819
GTGTAGA1950.044.8632542
GTGGTCG3000.041.99757454
GTAGATC2050.040.97052444
TGTACTC3150.040.0136152
CTAGGTG350.001255850939.9976950
CGTGTAG3600.038.88664216
CTCTAGT3250.038.7721336
AAGAGTG3550.038.45358326
TATAGTG2750.038.1770838