FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615611
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24010.14861250635208598No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC23440.1450844293583047Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT4500.059.0997237
GATAGGG4700.057.327636
TCGGTGG4800.056.87811751
TAGGGTG4900.053.55944438
GGGTGTA5250.051.32184640
GTAGGAT5400.051.2033432
TAGGATA5500.050.26925733
GTGTAGA5650.048.9286242
GGTGGTC5750.048.69832253
CGGTGGT5750.048.69832252
GGTCGCC5900.048.64974656
GTAGGGA6850.044.4522419
GGTGTAG6150.044.38031441
AGGATAG5950.042.34477634
GTAGATC5450.041.09808344
GTCGTGT7700.040.90880214
GTGGTCG6950.040.79360254
GAGTGTA7000.040.49971428
AGAGTGT7250.040.0686827
TAGATCT5450.039.81623545
Produced by FastQC (version 0.11.5)