FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771091
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14980.19427019638408438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC1400.035.0050956
TCGGTGG1550.033.873751
CGGTGGT1650.031.82074752
GGTGGTC1550.031.61750253
GTGCCTC1451.8189894E-1128.96221732
GTAGATC1700.028.82398444
GTAGGGA1850.028.37330419
GTCGCCG1950.026.92874157
GTGTAGA2000.026.24871342
GTCGTGT2150.026.04354314
TGTGTAG2050.025.60683841
AGTGTGC2100.024.9971529
CTAGTTG1601.8662831E-924.0566378
AGAGTGT1902.1827873E-1123.94463727
GTGGTCG2051.8189894E-1223.90591654
ATCTCGG2051.8189894E-1223.90436648
GCCTCTA2004.3655746E-1122.74593234
CCTCTAT1854.0381565E-1022.69864335
CTCTATG1905.638867E-1022.09987836
CGTGTAG2401.8189894E-1221.87250716
Produced by FastQC (version 0.11.5)