FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_surD_T2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_surD_T2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532968
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC10570.19832335149577462Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9240.1733687575989553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG2100.063.33485851
CGGTGGT2200.062.0469552
CTATAGT2350.056.58648737
GCTATAG2300.056.29514336
TGGGGTA252.3862626E-456.01711765
TAGTGTA2400.053.94951240
GGTGGTC2600.053.85756353
GGTCGCC2850.052.82316256
ATAGTGT2500.051.7915339
GTAGGGA3250.051.68385319
GAGTGTA2550.050.78077728
TGTAGGC2800.048.746631
GTCTAAC604.9149094E-946.6853181
GTGGTCG3150.046.68093554
CGTGTAG3550.045.34469616
AGAGTGT2900.044.65205827
GTAGGCT3000.043.16365432
GTCGCCG3100.042.91634457
TATAGTG2950.042.7048838
GTCGTGT3900.042.1725914
Produced by FastQC (version 0.11.5)