FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T4_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T4_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992496
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC29900.30126065999258433No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG28980.2919911012235818No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA25540.25733101191339813No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC24200.24382969805419868No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG22750.229220067385662No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC19390.19536602666408734No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA18060.181965468878464No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT17800.179345810965485No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG17780.17914429881833277No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT17140.1726959101094614No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA17020.171486837226548No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG16840.16967322790217795No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG16540.1666505456948945No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA16440.16564298495913332No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC14760.148715964598346Illumina Single End PCR Primer 1 (97% over 35bp)
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT13860.13964791797649562No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC13380.1348116264448421No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT13290.13390482178265706No Hit
GGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGC13230.13330028534120036No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA13220.13319952926762424No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA12500.12594509197014397No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG12480.12574357982299172No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT12410.12503828730795893No Hit
CGGCCATTAATCTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAG12370.12463526301365446No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA12220.12312392191001274No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC11980.12070577614418597No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGA11960.12050426399703375No Hit
CCGCCAGCTGCTACAGCGCGGCGCAATTCAAAAAGGCGAGGTTTCTTATA11360.11445889958246684No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG10630.10710370621141041No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT10610.10690219406425819No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA10500.10579387725492093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10260.10337573148909417No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA10220.10297270719478971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT3050.058.5156637
TCGGTGG3350.058.51032651
GTAGGAT3900.058.3332532
CGGTGGT3450.057.8289252
GGTGGTC3650.057.5370653
TAGGGTG3150.055.54987738
GGTGTAG3750.054.127841
GTGTAGA3800.053.41559642
GTGGTCG3950.053.1671654
GATAGGG3400.052.48934636
GGGTGTA3550.052.2481840
GGTCGCC4200.049.99992856
CGTGTAG4550.048.4565816
TAGGATA4550.047.69223433
CTCGGTG3500.047.00230450
GTAGATC3950.046.95722644
GTCGCCG4550.046.92537357
AGAGTGT4700.046.17247827
TAGATCT4050.045.80240245
GTAGGGA4850.045.46156319
Produced by FastQC (version 0.11.5)