FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765810
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC20740.270824355910735No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC19050.24875621890547264No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA17430.22760214674658205No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC16210.2116713022812447Illumina Single End PCR Primer 1 (96% over 33bp)
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG15190.19835207166268393No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT15000.1958710385082462No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT13850.18085425888928064No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA13810.18033193611992532No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG12960.1692325772711247No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG12870.16805735104007521No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT12520.16348702680821614No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA12440.1624423812695055No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC12120.1582637991146629No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA11950.15604392734490277No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG11850.15473812042151447No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT11230.14664211749650696No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT10670.13932959872553244No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG10540.13763204972512766No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA10390.1356733393400452No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA10360.13528159726302869No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA9670.12627152949164935No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT9470.12365991564487276No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC9190.12000365625938547No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG9050.11817552656664186No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG9020.11778378448962536No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA8900.11621681618155941No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8650.11295229887308862No Hit
CTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATG8510.111124169180345No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG8160.10655384494848592No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG7880.10289758556299865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG2400.052.50063751
GGTGGTC2750.050.91303353
GTGGTCG2750.050.91303354
CGGTGGT2700.047.96354352
TAGATCT2450.045.71185345
GGTCGCC3000.044.3367756
GTGTGCC2950.043.89597330
AGAGTGT3200.043.75053427
GTAGGGA3200.043.7448219
GTCGCCG3200.042.66234257
GTGCCTC3150.042.2199832
GTGTAGA3000.041.9950242
CCTCTAT3100.041.76924535
GTAGATC2850.041.7494444
CTCGGTG2800.041.250550
TACTACC1550.040.6403475
GTTACTA1400.039.9952623
TAGGGAA3500.039.99526220
AGATCTC2750.039.4524546
ATCTCGG2950.039.15046348
Produced by FastQC (version 0.11.5)