FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906125
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC23700.261553317698993No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT19310.21310525589736518No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA18830.2078079735135881No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA18300.2019588908815009No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC17940.1979859290936681No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA17350.19147468616360877No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA17210.18992964546834046No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT16670.18397020278659126No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA16140.17812112015450407No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG15850.17492067871430542No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA15180.16752655538694994No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA14840.16377431369844114No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG12850.1418126638156987No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA12310.13585322113394951No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC12300.13574286108428749No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG12080.133314939991723No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG11760.1297834184025383No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG11590.1279072975582839No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC11540.12735549730997378No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT11530.12724513726031175No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCG11270.12437577596909918No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG11250.12415505586977514No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA11130.12283073527383087No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC10920.1205131742309284No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCT10520.11609877224444752No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA10410.11488481169816528No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG9540.10528348737756932No Hit
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA9500.10484204717892123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT800.052.4972737
TCGGTGG1400.042.50248351
GTCGTGT1800.040.8334614
GTAGGGA1400.040.00012619
CGGTGGT1500.039.6689852
GTGGTCG1650.038.18404854
ACTAGTA601.2166964E-534.99817737
CACTAGT601.2176168E-534.99431636
GGTGGTC1750.034.00198453
GTGGAGA3850.033.6494671
TAGTGTA1357.2759576E-1231.1112140
TACCCAC450.004309081531.10949329
TATAGTG1500.030.33342738
GGTCGCC2200.030.22903456
ATAGTGT1401.2732926E-1130.00009539
AGCGTCG2500.029.39684711
TCTAGTT2500.029.3968477
GTCTAAC1202.2919266E-929.178031
CTCTAGT2400.029.1635386
GCTATAG1451.8189894E-1128.96081536
Produced by FastQC (version 0.11.5)