Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_PY79_T3_R3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 854364 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG | 1893 | 0.22156832450805514 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 1494 | 0.1748669185499389 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 1358 | 0.1589486448399043 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 1174 | 0.13741215687926925 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 993 | 0.11622680730929674 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC | 941 | 0.11014040853781293 | Illumina Single End PCR Primer 1 (97% over 35bp) |
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG | 896 | 0.10487333267787499 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATAGGGT | 215 | 0.0 | 55.350826 | 37 |
CGGTGGT | 265 | 0.0 | 54.14972 | 52 |
GATAGGG | 235 | 0.0 | 50.63123 | 36 |
CTCTAGT | 460 | 0.0 | 50.216255 | 6 |
GGTGGTC | 280 | 0.0 | 50.001797 | 53 |
GTGGTCG | 305 | 0.0 | 49.346035 | 54 |
TCGGTGG | 285 | 0.0 | 49.121696 | 51 |
GTAGGAT | 315 | 0.0 | 47.776695 | 32 |
GTGTAGA | 280 | 0.0 | 47.498928 | 42 |
ACTCTAG | 495 | 0.0 | 46.668343 | 5 |
GGTCGCC | 340 | 0.0 | 46.325195 | 56 |
TAGGATA | 320 | 0.0 | 45.933773 | 33 |
AGAGTGT | 315 | 0.0 | 45.554523 | 27 |
GTAGATC | 250 | 0.0 | 43.39902 | 44 |
TCTAGTT | 545 | 0.0 | 43.02151 | 7 |
GTCGTGT | 425 | 0.0 | 42.820057 | 14 |
TGTACTC | 560 | 0.0 | 41.27081 | 2 |
TAGGGTG | 285 | 0.0 | 40.5254 | 38 |
AGATCTC | 260 | 0.0 | 40.383705 | 46 |
GTAGGGA | 365 | 0.0 | 40.270702 | 19 |