FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T3_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T3_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854364
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG18930.22156832450805514No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA14940.1748669185499389No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT13580.1589486448399043No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC11740.13741215687926925No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA9930.11622680730929674No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC9410.11014040853781293Illumina Single End PCR Primer 1 (97% over 35bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG8960.10487333267787499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT2150.055.35082637
CGGTGGT2650.054.1497252
GATAGGG2350.050.6312336
CTCTAGT4600.050.2162556
GGTGGTC2800.050.00179753
GTGGTCG3050.049.34603554
TCGGTGG2850.049.12169651
GTAGGAT3150.047.77669532
GTGTAGA2800.047.49892842
ACTCTAG4950.046.6683435
GGTCGCC3400.046.32519556
TAGGATA3200.045.93377333
AGAGTGT3150.045.55452327
GTAGATC2500.043.3990244
TCTAGTT5450.043.021517
GTCGTGT4250.042.82005714
TGTACTC5600.041.270812
TAGGGTG2850.040.525438
AGATCTC2600.040.38370546
GTAGGGA3650.040.27070219