Basic Statistics
Measure | Value |
---|---|
Filename | HLNTYAFXY_n02_PY79_T3_R2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 598129 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC | 7301 | 1.2206396947815605 | Illumina Single End PCR Primer 1 (96% over 33bp) |
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC | 868 | 0.14511919669502732 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 801 | 0.1339175997151116 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 750 | 0.12539101096920566 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 739 | 0.12355194280832396 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATA | 733 | 0.12254881472057032 | Illumina Single End PCR Primer 1 (96% over 33bp) |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 725 | 0.12121131060356545 | No Hit |
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC | 665 | 0.111180029726029 | No Hit |
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG | 649 | 0.10850502149201927 | No Hit |
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA | 639 | 0.10683314134576322 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 637 | 0.10649876531651199 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 627 | 0.10482688517025592 | No Hit |
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG | 609 | 0.10181750090699497 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 606 | 0.10131593686311816 | No Hit |
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT | 605 | 0.10114874884849254 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGGTC | 1295 | 0.0 | 67.305336 | 53 |
CGGTGGT | 1240 | 0.0 | 66.90316 | 52 |
GGTCGCC | 1365 | 0.0 | 66.41819 | 56 |
TCGGTGG | 1220 | 0.0 | 66.278404 | 51 |
GTGGTCG | 1350 | 0.0 | 66.11901 | 54 |
CTAGGTG | 85 | 0.0 | 65.89022 | 50 |
GTGTAGA | 1140 | 0.0 | 65.698616 | 42 |
GCCTCTA | 1285 | 0.0 | 64.81639 | 34 |
CCTCTAT | 1350 | 0.0 | 64.806305 | 35 |
GTGCCTC | 1315 | 0.0 | 63.60381 | 32 |
GTAGATC | 1110 | 0.0 | 63.37535 | 44 |
GTGTGCC | 1365 | 0.0 | 63.325012 | 30 |
TGTAGAT | 1265 | 0.0 | 62.80332 | 43 |
GTCGCCG | 1400 | 0.0 | 62.7575 | 57 |
CCCTATC | 140 | 0.0 | 62.50747 | 61 |
CTCTATG | 1245 | 0.0 | 62.40144 | 36 |
TCGCCGT | 1425 | 0.0 | 61.902134 | 58 |
AGTGTGC | 1400 | 0.0 | 61.74189 | 29 |
AGATCTC | 1085 | 0.0 | 61.620262 | 46 |
TAGATCT | 1165 | 0.0 | 61.595345 | 45 |