FastQCFastQC Report
Tue 16 Jul 2019
HLNTYAFXY_n02_PY79_T3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNTYAFXY_n02_PY79_T3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598129
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC73011.2206396947815605Illumina Single End PCR Primer 1 (96% over 33bp)
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC8680.14511919669502732No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA8010.1339175997151116No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT7500.12539101096920566No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC7390.12355194280832396No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATA7330.12254881472057032Illumina Single End PCR Primer 1 (96% over 33bp)
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA7250.12121131060356545No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC6650.111180029726029No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG6490.10850502149201927No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA6390.10683314134576322No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA6370.10649876531651199No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT6270.10482688517025592No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG6090.10181750090699497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6060.10131593686311816No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT6050.10114874884849254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC12950.067.30533653
CGGTGGT12400.066.9031652
GGTCGCC13650.066.4181956
TCGGTGG12200.066.27840451
GTGGTCG13500.066.1190154
CTAGGTG850.065.8902250
GTGTAGA11400.065.69861642
GCCTCTA12850.064.8163934
CCTCTAT13500.064.80630535
GTGCCTC13150.063.6038132
GTAGATC11100.063.3753544
GTGTGCC13650.063.32501230
TGTAGAT12650.062.8033243
GTCGCCG14000.062.757557
CCCTATC1400.062.5074761
CTCTATG12450.062.4014436
TCGCCGT14250.061.90213458
AGTGTGC14000.061.7418929
AGATCTC10850.061.62026246
TAGATCT11650.061.59534545